The spread of antibiotic resistance genes (ARg) is a worldwide health risk1 and is no longer only a clinical issue. Vast reservoirs of ARg are found in natural environments2–4 such as soils, sediments and oceans. The emergence and release of ARg to the environment is in particular caused by extended use of antibiotics in farming, e.g. where the genes dissipate from the manure.5 Once in the environment, the ARg are surprisingly rapidly propagated. It is well known that the ARg are distributed to neighbour bacteria through processes of both cell sharing or through horizontal gene transfer (HGT) where one species acquirer resistance from another.6,7 Most HGT responsible for the spread of ARg are assumed to be through direct microbe-microbe contact. However, I find that the outcome of non-contact transfer is grossly underestimated. In the HGT mechanism called “Transformation”, free ARg in suspension or adsorbed to a mineral can be picked up and incorporated into non-related organisms. Considering that free DNA only can survive for a few weeks in sea- and freshwater environments,8–10 any HGT from free DNA can rightly be assumed to be local, but if the DNA gets adsorbed to a mineral, it can survive for several hundred thousands of years.11–14 If this also holds for ARg, then minerals offer a potent mechanism for distributing ARg through our environments my means of sedimentary processes.
Updated: Feb 2, 2021
Christiane is training Ioannis in extraction of modern DNA from sediments.
We are setting up and testing extractions protocols to address DNA-mineral interactions observed at the nanoscale.
The efforts are part of Ioannis´ DENMARK project and the extraction procedures are carried out in collaboration with Antonio Fernandez Guerra and Marina Zure.
A fascinating look into the technical process of DNA extraction and the importance of precision in scientific work. Complex research projects require structured planning, collaboration, and careful execution at every stage. Professionals may also benefit from a premier strategic project management course & workshop for managers in Copenhagen, Denmark to strengthen long-term planning and project leadership skills. Thank you for sharing this informative article.
This is a thoughtful and engaging piece. The discussion on setting up DNA extractions and bridging nano- to bulk-scale processes clearly highlights the importance of precision, coordination, and scalability in scientific work. It helps readers understand how complex research efforts depend on structured planning and seamless execution. This topic also connects well with ideas explored in a strategic project management seminar & course for executives in Copenhagen, Denmark, where professionals learn how to manage high-complexity projects, align technical workflows, and ensure efficient delivery of outcomes across multidisciplinary environments.